"My decision was due to a whole set of circumstances. I believe this is the best decision. There are a lot of reasons, but I don't want to name them because they are interpreted differently," he told BNS.
Pulkauninkas would not comment on whether he received more political support following his first letter of resignation which was rejected by the minister in June.
The chief forest officer said the minister's approval is not required for unilateral termination of his employment contract.
In his first resignation letter, he sought termination of the employment contract by mutual agreement.
In June, Pulkauninkas said he felt that he had no political support in his fight against interest groups in the wood industry.
He acknowledged that his resignation was also due to the government's decision to temporarily allow selling wood from state forests at a 20 percent discount, a move that the chief forest officer said might cost the state 68 million euros.
The 41-year-old man has served as chief executive officer of Valstybiniu Misku Uredija (State Forestry Enterprise) since March 2018.